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Environmental DNA, RNA key in monitoring Covid pathogens: Study

New York, April 21 (IANS) Sampling environmental DNA and RNA (eDNA and eRNA) can better help determine the presence of both human and wildlife pathogens, according to researchers.

The eDNA and eRNA approach works through the collection of a sample (often from an aquatic source), whose genetic contents are then sequenced to reveal the presence and prevalence of pathogens.

This eDNA or eRNA can give researchers a timely view into disease spread, which “can help predict the spread of pathogens to nearby new and susceptible geographic locations and populations in advance, providing opportunities to implement prevention and mitigation strategies,” said a team of researchers, including Jessica Farrell from the University of Florida.

For instance, researchers used eRNA analysis of wastewater to track large-scale outbreaks of the Covid-19 disease. They found that “wastewater detection of SARS-CoV-2 eRNA increased rapidly prior to medical detection of human outbreaks in those regions, with environmental virus concentration peaking at the same time or before the number of human-detected cases, providing advanced warning of a surge in infected individuals.”

With this advanced knowledge, crucial and limited medical resources can be provisioned where they will be most needed, the team wrote in the journal BioScience.

The benefits of eDNA and eRNA analysis are not limited to the detection of human pathogens; the authors describe the ways in which these tools also help in understanding the presence and transmission of pathogens that hamper wildlife conservation efforts, such as the turtle-specific DNA virus, chelonid herpesvirus 5.

eDNA monitoring of this pathogen may help researchers evaluate the disease’s spread — in particular, the idea that the virus is most frequently transmitted by “superspreader” individuals.



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